+44 1625708989Development of UV Visible Spectrophotometric Method for Estimation of Cefipime Hydrochloride in Bulk and Dosage Form
Author(s): Sathyanarayana L, Samatha K*, Susmitha K, Sanath kumar KThe three simple, accurate, rapid, and sensitive UV-Visible spectrophotometric methods have been developed for estimation of Cefepime Hydrochloride in bulk and pharmaceutical formulation. It describes development of 3 new visible spectrophotometric based on different chemical reactions namely cefepime hydrochloride and reagent (1,10 Phenanthroline α-napthylamine (BM reagent). Among 3 methods CFP1 (512 nm) and CFP2 (565 nm) more sensitive followed by CFP3 (510 nm) because of higher λmax compared to methodsCFP1 and CFP2. The proposed 3 methods are based on exploitation of 3 structural feature of molecule (Aromatic amino group and oxidized nature of CFP in developing simple and sensitive method for assay of Cefepime hydrochloride. The result of Analysis of pharmaceutical formulation reveals that the proposed methods are suitable for Analysis. All these methods were used to produce coloured species. The methods were extended for determination of Cefepime hydrochloride in parenteral preparation was chosen. All the proposed methods are simple and reliable and provide a wide choice for determination of Cefepime hydrochloride in bulk or in pharmaceutical formulation depending on specific analytical situation
PDF