Wounds are common clinical entities of life which may be subacute or acute. Wound healing is a complex biochemical process where the cell structures are restored to normalcy, which depend on cell proliferation and migration, basically fibroblast cell.Plant polyphenols initiate wound healing process in natural way. The aim of this study was to evaluate the wound healing effect of standardized brazilin rich extract (BRE) from Casealpiniasappan heartwood. In vitro cell proliferation and migration of human fibroblast cell were determined by using scratch wound assay technique. Cytotoxicity, antioxidant and antibacterial activity of brazilin and BRE were also evaluated. Result indicated that brazilin and BRE had showed similar antioxidant and antibacterial activity (P > 0.05). Cytotoxicity study revealed that brazilin or BRE was nontoxic up to 500 μg/mL concentrations. Therefore, BRE can be used as potential bioactive replacer for brazilin owing to ease of production. Invitroscratch wound assay showed that BRE at 250 μg/ mL had highest cell proliferation and migration on day 2 of incubation. BRE treatment resulted in restoration of original cell number within 2 days when compared to control (P< 0.05). Hence, BRE could be used as a potential herbal compound for the treatment of wound healing. BRE could be used as a replacer for brazilin owing to its similar biological activities and ease of low cost production.