Flavonoids in the diet useful for the control of stress which plays an essential role in control of the development of cardiomyopathy in doxorubicin treatment on cancer therapy. The present study represents the protective effect of vitamin –E, flavonoids morin, rutin and quercetin on heart, liver and kidney homogenate biochemical parameters such as protein, sugar, urea, blood urea nitrogen, creatinine and phosphorus. Treatment of experimental rabbits with flavonoids and vitamin-E for 28 days and two doses of doxorubicin on 29th and 30th day, than the animals were sacrificed. The tissues of heart, liver and kidneys were collected and tissue homogenates were prepared by using phosphate buffer saline solution. The homogenate examined for biochemical parameters protein, sugar, urea, creatinine and phosphorus by standard methods. The values were tested with Dunnette multiple significant test P<0.01 and P<0.05. The results of this study strongly indicate that flavonoids had the protective effect against doxorubicin induced oxidative stress in the experimental rabbits. This study also suggests that rabbits were good animal model for the experiments in the pharmacology and biochemistry.