In the present study UV-Spectrophotometry and RP-HPLC methods were validated for the simultaneous analysis of acetaminophen in marketed tablets. The methods were validated in terms of linearity, sensitivity (Detection limit and Quantification limit) accuracy (% Recovery), precision (inter day, intraday and reproducibility). Both the methods were linear (r2 = 0.9993 for UV method and 0.9995 for HPLC) and accurate (% recovery was 99.48 % - 101.42 % for UV method and 101.85 % - 102.35 % for HPLC method). The detection limit and quantification limit were 0.192 μg/ml and 0.640 μg/ml for UV method and 0.0155 μg/ml and 0.0518 μg/ml. The method was also found precise (% RSD < 5%) and robust. Assay of five marketed brands of paracetamol were determined by both the methods and no statistically significant difference was noticed between the assay obtained from UV-Spectrophotometry and RPHPLC methods by paired t - test at 5 % significance level. The results obtained from the mean percentage analysis of paracetamol tablets (%) containing 500 mg of acetaminophen shows that the mean percentage determined in three replicate analyses is more than the claimed amount by the manufacturers. The two methods were found to be linear, quantitative, reproducible and could be used as a more convenient, efficient and economical method for the trace analysis of drug in raw material, tablets and in biological fluids.