Original Articles: 2010 Vol: 2 Issue: 3
UV-Spectrophotometric estimation of Diacerein in pharmaceutical formulation
Abstract
Three simple, precise and economical UV methods hav e been developed for the estimation of Diacerein in Pharmaceutical Formulations. Diacerein has the maximum absorbance at 256.2 nm in zero order spectra formed the basis for metho d A while first order derivative spectrum showed peak at 250.0 nm when n = 1 for method b and method C applied was Area under Curve (AUC) in wavelength range of 262.0-250.0 nm.. Metho d A utilises A1%, 1cm value at λ max for its analysis. Calibration curve (Regression equatio n) was used for method B and C for analysis of Diacerein respectively. Drug was found to obey t he Beer-Lambert’s law in the concentration range of 5-30μ g/mL in all three proposed methods. R esults of the analysis were validated statistically and by recovery studies. Results were found to be satisfactory and can be adopted for routine analysis of the drug