Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2010 Vol: 2 Issue: 1

UV- Spectrophotometric determination of Ceftazidime in pure and pharmaceutical formulation

Abstract

A simple sensitive spectrophotometric method in UV region has been developed for the determination of Ceftazidime in bulk dosage form. The solution of ceftazidime in 0.1N HCl shows maximum absorbance at 261nm, beer’s law was o beyed in the concentration range of 2-10 μ g ml -1 . The absorbance was found to increase linearly wit h increasing concentration of ceftazidime, which is corroborated by the calculate d correlation coefficient value of 0.9981. The slope and intercept of the equation of the regressi on line are 0.0465 and 0.0007 respectively. The analysis were validated statistically and its r ecovery studies result of percentage shows that the method was not affected by the presence of exce pients which proves suitability of the developed method for the routine estimation of ceft azidime bulk and solid dosage form. This method were extended to pharmaceutical formulations and there no interference from excepients and diluents. The proposed methods are economical a nd sensitive for the estimation of ceftazidime in bulk dosage form.