Simple and precise UV spectrophotometric methods by second and third order derivative have been developed and validated for the estimation of fexofenadine hydrochloride in bulk and its tablet formulation. The standard and sample solutions of fexofenadine hydrochloride were prepared in 0.1 N Hydrochloric acid. Fexofenadine hydrochloride was estimated at 215 nm for the second order derivative and 233.7 nm for third order derivative respectively. Beer’s law was obeyed in the concentration range of 1 to 14 μg / ml with coefficient of correlation values were 0.9992 for second order derivative method and 0.9997 for third order derivative method respectively. These methods were tested and validated for various parameters according to ICH guidelines. The precision expressed as relative standard deviation were of 0.8908 % and 1.152% for the above two methods respectively. The proposed methods were successfully applied for the determination of fexofenadine hydrochloride in pharmaceutical formulation. Results of the analysis were validated statistically and were found to be satisfactory. The proposed methods are simple, easy to apply, low-cost and require relatively inexpensive instruments.