The molecular interactions in the binary mixtures were analyzed by ultrasonic measurements using interferometric method. The experimental data of density, ultrasonic velocity(U), intermolecular free length(Lf), specific acoustic impedance(Z), relative association(RA) of substituted azomethine in binary mixture (70% DMF+ water) over the entire composition range at 300K have been investigated. The results obtained in this study have been interpreted in terms of different interactions among solute–solute and solute–solvent.