Chemical investigation of the bioactive constituents from the leaf of a versatile Nigerian medicinal plant Bryophyllum pinnatum resulted in the isolation of two new novel flavonoids; 5I Methyl 4I, 5, 7 trihydroxyl flavone 1 and 4I, 3, 5, 7 tetrahydroxy 5-methyl 5I-propenamine anthocyanidines 2. The structure of these compounds were elucidated using NMR spectroscopy in combination with UV, IR and MS spectra data. Antimicrobial studies showed that the isolated compounds successfully inhibited Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli, Staphylococcus aureus, Candida albicans and Aspergillus niger. The antimicrobial observation of the above compounds could be responsible for the activity of Bryophyllum pinnatum and its use in herbal medicine in Nigeria.