Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

header
Reach Us reach to JOCPR whatsapp-JOCPR +44 1625708989
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Original Articles: 2014 Vol: 6 Issue: 5

Transformation of green fluorescent protein gene in Valsa mali var.mali using Agrobacterium tumefaciens

Abstract

Valsa mali var. mali is the causal agent of apple tree canker disease, causing severe economic losses in major apple production areas in China. To better understand the infection mechanisms of V. mali and plant-pathogen interactions, the pathogen was genetically transformed with enhance green fluorescence protein (EGFP) as a vital marker using Agrobacterium tumefaciens-mediated method. The average transformation efficiency was 675.00±20.61 transformants per 106 recipient conidia, and transformants showed mitotic stability and EGFP expression. This is the first successful transformation of V. mali with EGFP expression, allowing hyphal visualization in vivo.