Botnets have become the most serious security threa ts on the current Internet infrastructure. Botnets can not only be implemented using existing well known applications, but also be constructed by unknown or creative app lications. This makes the botnet detection a challenging probl em. P2P botnets with low resource requirements have developed rapidly. In this paper, a novel P2P node-based dete ction is proposed, which exploits the node profile of the novel behaviors. Our experimental results show that it no t only successfully detects known P2P botnets with a high detection rate, but also detects some unknown P2P m alware.