Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2015 Vol: 7 Issue: 6

The estimation of Ketorolac drug in Ketorolac Tromethamine eye dropsby RP-HPLC


A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Ketorolac drug in Ketorolac Tromethamine eye drops. An Alltech Spherisorb C8 150 mm x 4.6 mm, 5 μ with mobile phase consisting of Water, methanol and glacial acetic acid were mixed in the ratio 44:55:1 (v/v/v) respectively and degassed for about 10 minutes. Isocratic mode of chromatography technique was used. The flow rate was 1.2 mL/min and the eluents were monitored at 254 nm. The retention time of the Ketorolac peak is about 3 minutes. The detector response was linear in the concentration of 0.015 - 2.999 mcg/mL. The respective linear regression Yintercept is 111.314, Residual sum of square is 310322.267 and slop is 44538.321. The limit of detection and limit of quantification were 0.0049 mcg/mL and 0.0150 mcg/mL respectively. The percentage assay of Ketorolac was 99.9 %. The method was validated by determining its accuracy, precision and linearity.