Frankincense resin is used for treating many diseases in traditional medicine. This resin is prepared from Boswellia carteri plant. The goal of this study was evaluating the effects of hydroalcoholic extract of Frankincense on pituitary- gonadal axis of mice. Mice were divided into five groups including control, placebo, and three experimental groups with 10 members in each group. Control group did not receive any drug and experimental groups received intraperitoneal injections of 50, 100, and 200 mg/mg of the extract for 20 days every other day. Blood samples were taken at the end of injection period. FSH, LH, estrogen and progesterone hormones were measured. Obtained results were analyzed using SPSS program in a one way analysis of variance and means were compared using Duncan test at 5% probability level. According to results, Frankincense decreased the testosterone hormone in 200 mg/kg dose. FSH amount was increased in 100 and 200 mg/kg groups. Considering the results, frankincense extract can reduce reproductive potential by reducing sexual hormones in male mice dose dependently.