In this paper, the carbon nanotube was applied to prepare an appropriate bed for immobilization of Glucose Oxidase enzyme. Numerous operational advantages of glucose oxidize enzyme matrix may include: efficiency and more accurate control over enzyme reaction, protection against destruction and waste of enzymes, the use of matrix enzyme have a high efficiency and more control of enzymic reactions. For immobilization and stabilization of enzyme on the bed, the prepared matrix was washed by sulfuric acid, nitric acid and enough ionized water. The matrix surface, for restrain distort enzyme, was covered by Sistamine. The glucose oxidize enzyme was connected on the matrix by intermediate material; one side of intermediate material is amid group and another side is pyren group. For elimination of impurity, the prepared matrix was placed into Dimethylformamide (DMF) for several hours. The activity parameter of enzyme was measured by Spectrophotometer in the 460 nm wave length; the immobilized enzyme could be stabilized for long time. This matrix could be used as an electrode in sensors structure.