The vast rural areas in China are now facing increasing problems and conflicts with the fast development of the economy and society, especially the rapid advancement of the urbanization process. Finding the way to shift the urban-rural dual structure to urban-rural integration is in desperate need. Urban and rural planning can not only serve as a tool of practicing development policies but can also provide basis for policy-making. The paper takes Jiangning District, which is located at the urban and rural junction region of Nanjing as an example to make spatial analysis through the application of mapinfo 9.5 based on neural network theory and the spatial analyzing approaches of the urban-rural integrated planning. Meanwhile, the paper makes a couple of beneficial discoveries in areas including equilibrium of urban and rural land use, layout of the rural industries, distribution of basic infrastructures, protection of the ecological environment and the innovation of the policy-making mechanisms.