A new series of triorganotin(IV) complexes of monofunctional bidentate Schiff base have been synthesized and characterized through elemental analysis, conductance measurements, molecular weight determinations, UVvisible, multinuclear (1H, 13C, 119Sn) NMR spectroscopy, FT-IR, X-ray powder diffraction and theoretical calculations. On the basis of these techniques, it is proposed that the ligands are bounded to the tin atom through the azomethine nitrogen and carboxylate oxygen. The data reveal that triorganotin(IV) complexes have pentacoordination with trigonal bi-pyramidal geometry. Molecular modeling has been provided in support of the geometry of complexes. The synthesis organotin(IV) compounds have been tested against various Gram-positive and Gram-negative bacteria (Bacillus cereus, E. coli, Klebsiella spp. and Staphylococcus spp.). The results obtained show that the synthesized organotin(IV) complexes have promising activity against all the tested microorganisms.