Five new azo dyes for acridine derivatives were synthesized using different approaches depending on the position of functional groups. The synthesis started with a commercially available unsubstituted acridine that was converted by nitration and amination to compounds that was useful building block in supramolecular chemistry by diazotization of 4(2,4-dichloro-δ-triazin)aniline DCTA . the structures of newly synthesized compounds were characterized by NMR, Mass, FT-IR spectra data and elemental analysis. All the synthesized compounds were screened for their antibacterial activities.