Cyclohexenone derivative (2) prepared by reaction of butanoic acid (1) with ethyl acetoacetate reacts with aromatic aldehydes, hydrazine hydrate, thiosemicarbazide to give arylidene, indazole and thiotriazole derivatives. The behavior of the obtained thiotriazole towards hydrazine hydrate, aromatic amines, sodium nitrite, acetylacetone, anthranilic acid, thiourea, benzalacetophenone has been studied. The structure of all synthesized compounds were confirmed by micro analytical and spectral data. The antitumor activity of some of the synthesized compounds were tested .