We present the synthesis of spherical silver nanopa rticles by simple and low cost chemical route. Well-dispersed silver nanoparticles were prepared b y reducing silver nitrate (AgNO 3 ) with glucose in presence of protective agent polyvinyl p yrrolidone (PVP). Sodium hydroxide was used to enhance the reaction velocity. The prepared nano particles were characterized by X-ray diffraction (XRD), scanning electron microscopy (SE M) and transmission electron microscopy (TEM). XRD pattern showed the face centered cubic s tructure of silver nanoparticles. The crystallite size was found to be 18 nm using Scherr er formula. The spherical morphology of silver nanoparticles was confirmed from SEM image. The transmission electron microscopy (TEM) image showed the average particle size of sil ver nanoparticles was about 15 nm. Silver nanoparticles can kills bacteria when spreads on sh oes, socks, phone and even computer keyboard. A preliminary test shows that silver nano particles are highly active against S. aureus , E. coli , and P. aeruginosa. This reveals that silver nanoparticles could provide a safer alternat ive to conventional antimicrobial and antibacterial age nts.