Novel heterocyclic compound containing pyrimido-pyrimidine moiety have been synthesized by the reaction of 2,4,6,8-tetrachloropyrimido[5,4-d]pyrimidine (DBMY-2) with sequential nucleophillic substitutions of Piperazine, diethanolamine, diethanolamine and ethanolamine in the pattern of C-4,C-8,C-2 and C-6 respectively. The use of low temperature, relatively dilute solution, and careful addition of amine nuleophile can control the critical steps, which have been characterized by using IR and 1H NMR and mass spectral data.