2-substituted-N'-(1-(4-hydroxycumarinyl) ethylidene)benzohydrazide were synthesized by condensation of 2-substituted benzohydrazide with 3-acetyl-4-hydroxycumarine. The structure of each was established by IR, 1HNMR, 13CNMR and mass spectral studies. Antibacterial studies of these compounds were tested in vitro against E. coli, S. typhi, S. aureus, and B. subtilis by agar cup method. Antifungal studies were tested against A. niger, A. flavus, P. notatum and F. moneliforme by poison plate method.