The new Ru (II) complex [Ru(py)4(dppz)]2+ (dppz = dipyrido phenazine) have been synthesized and characterized by different analytical techniques like elemental analysis, LC-MS, 1H NMR , 13C NMR. The interaction of this complex with calf thymus DNA has been explored by electronic absorption spectroscopy, Fluorescence measurements, viscosity measurements. Their photocleavage behaviour towards pBR 322 and anti microbial properties of this complex was also investigated. The experimental results show that the complex binds with DNA by intercalation mode.