Using 4-chlorobenzohydrazide as a source of amine and substituted aromatic aldehydes as a carbonyl source, concentrated hydrochloric acid as catalyst at room temperature 4-chlorobenzohydrazide derivatives was synthesized. The compounds (S5-S6) were characterized by FT-IR, 1H NMR and 13C NMR. The compounds were screened for antimicrobial activity against S. aureus, E. coli and A. niger. The antioxidant activities of synthesized Schiff bases were investigated using the DPPH radical scavenging method. The designed compounds were further subjected for molecular docking study by in silico method to analyse anti-tuberculosis against InhA, the enoyl acyl carrier protein reductase (ENR) from Mycobacterium tuberculosis by Discover studio 2.1version software.