The present study deals with a biologically important transition metal complexes. The complex containing Cu(II), Co(II), Mn(II) and Ni(II) ions were synthesised by using 2,2-diphenylethanamine and 2-hydroxy-4-methoxy benzophenone. The ligand and complexes were characterised separately by microanalytical, IR, NMR, UV-Visible, Cyclic voltammetry and the EPR spectroscopic techniques. The spectral data confirm the ligand acts as a neutral bidendate Schiff base, coordinating through azomethine nitrogen and oxygen atom of hydroxyl group. The interaction studies of these complexes with CT-DNA have also been performed by using spectral and electrophoresis techniques collectively indicated of the evidences for groove binding of DNA with metal complexes. In addition, the complexes showed their efficient antimicrobial activities against bacteria (Escherichia coli & Staphylococcus aureus).