The solid complexes of Zn(II), Pd(II) and Ru(III)of ethylenediamine bis dehydroacetic acid hydrazone were synthesized and characterized by elemental analysis, conductometry, thermal analysis, magnetic measurements, IR, 1H-NMR and UV––Vis spectroscopy and a biological studies. From the analytical and spectral data, the stoichiometry of the complexes was found to be 1:1 (metal: ligand). The physic-chemical data suggest an octahedral geometry for the Ru(III) and Zn(II) complexes and a square planar for Pd(II) complex. The thermal decomposition of all the complexes was studied by the TG–DTA method. The synthesized ligand and its metal complexes were screened for their in vitro antibacterial activity against Gram-negative (Pseudomonas aeruginosa & Sarcina SP) and Gram-positive (Micrococcus lutes&Bacillus Subtilis) bacterial strains and for in vitro antifungal activity against Aspergillus niger and Candida albicans. The results of these studies showed that the [Pd(HL)] Cl . 4H2O complex have antibacterial and antifungal activities than the free ligand and other complexes. The cytotoxicity of the ligand (H2L) and its complexes on human liver carcinoma HEPG2 and breast carcinoma cells lines MCF7were determined. The Pd(II) complex showed a significant more cytotoxicity activity with a lower IC50 value of 2.67 ug/ml compared to the other complexes moreover the Pd(II) complex more active than the reference drug (IC50=3.73 ug/ml) therefore we believed to possess a significant role in the medicinal area in the future.