The synthesis of Cu(II) and Ni(II) complexes with thiosemicarbazone(L) ligand have been reported. The ligand was characterized by elemental analysis and spectral (IR and 1H NMR) studies and its complexes were characterized by electronic, UV/VIS, EPR and IR studies. At room-temperature EPR spectrum of Cu(II) complex was recorded, indicated distorted octahedral structure. The electronic spectrum of Ni(II) complex indicated the octahedral geometry around the Ni(II) ion. Their antibacterial activities were evaluated by the diameter of zone of inhibition (mm) against four bacteria such as B. subtilis, S. aureus, E. coli and K. pneumonia. The ligand and the 6-coordinate Ni(II) complex did not inhibit the growth of the test organisms, while the Cu(II) complex showed good antibacterial activity than parent ligand against the same bacteria