The aim and objective of the work was to develop novel thiazole-1, 3, 4-oxadizole hybrid analogues and to evaluate their invitro cytotoxic activity. In this study, 9 novel thiazole-1,3,4-oxadiazole hybrid analogues were synthesized by cyclodehydrogenation reaction of 2-[(4-phenyl-1,3-thiazol-2-yl)amino]acetohydrazide with substituted aliphatic or aromatic acids using phosphorusoxychloride as dehydrating agent to yield N-[(5-substituted-1,3,4-oxadiazol-2- yl)methyl]-4-phenyl-1,3-thiazol-2-amine. The synthesized compounds were then established on the basis of IR,1HNMR spectral datas and screened for invitro anticancer activity on human breast cancer cell line MCF-7 and lymphoma cancer cell line DLA . The derivatives showed moderate activity on both cell lines.