The Schiff base complexes of Co(II), Ni(II), Cu(II) and Zn(II) derived from 3-pyridine carboxaldehyde and Ltryptophan. They have been synthesized and investigated by different physicochemical techniques. The resulting complexes were characterized by elemental analysis, molar conductance, IR, XRD and SEM. The low conductance values indicate that all the complexes are non-electrolytes. The metal in the Schiff base complex is hexacoordinated binds through pyridine nitrogen, azomethine nitrogen and nitrate units of metal salt used in the preparation. The synthesized compounds were tested for antimicrobial activity against the bacteria Klebsiella pneumonia, Pseudomonas aeroginosa, E.Coli, Staphylococus aureus, Proteus and Aspergillus niger. The complexes exhibit higher antimicrobial activity than the free Schiff base because of chelation.