As a part of systematic investigation of synthesis, characterization and biological activity of several new substituted N-benzhydrylpiperidin-4-amine derivatives (7a-7j) have been synthesized by reductive amination of Benzhydrylamine and N-Substituted 4-piperidone (3a-3e) as the starting material. . The structures of all the synthesized compounds have been determined by their spectral and microanalytical data. All the synthesized products were evaluated for their antibacterial activity against Bacillus substilis, Escherichia coli. Klebsiella pneumoniae and Streptococcus aureus bacteria and antifungal activity against Aspergillus niger, Aspergillus flavus, fungi respectively. Most of the synthesized compounds exhibited significant anti-bacterial and anti-fungal activities.