Heterocyclic Compounds having a valuable place in a Heterocyclic Chemistry and Heterocyclic Compounds having a excellent properties such as drugs, dyes etc, This compounds are showing anti microbial, anti fungal, anti bacterial, anti inflammatory,anti diabetic, anti hypertensive etc. properties. In present investigation, we have prepared N-[{4-[2-{5-(4-chlorophenyl)-3-(4-methoxyphenyl)-4,5-dihydro-pyrazol-1-yl}-2-oxoethoxy] phenyl} methylene] substituted aniline from chalcon of 3-(4-Chlorophenyl)-1-(4- methoxy phenyl)prop-2-en-1-one and Schiff base of 2-(4-{[(substituted phenyl) imino]methyl} phenoxy)acetohydrazide. Compound having a excellent properties regarding as per as anti cancer and HIV as compare to this compound. Physical properties of pure crystallized substance N-[{4-[2-{5-(4-chlorophenyl)-3-(methoxyphenyl)-4,5-dihydro-pyrazol-1-yl}-2-oxo ethoxy] phenyl}methylene ]substituted aniline like M.P elementary analysis and spectral data of compound and such as IR and NMR will be evaluated and confirm the structure of compound. All the synthesized products were evaluated for their antimicrobial activity. All the compounds were tested for their antibacterial and antifungal activities by broth dilution method.