Some N-[(2-oxo-3-chloro-4-substituted phenyl-azetidineimino)-propyl] derivatives bearing indole moieties have been synthesized and evaluated in vitro for their antitubercular activity against Mycobacterium tuberculosis H37Rv and antimicrobial activity against a panel of bacteria and fungi. All these compounds were fully characterized by spectroscopic methods and elemental analysis. Some of the tested compounds showed moderate to excellent antitubercular and antimicrobial activities.