La (III), Sm (III), Th (IV) and UO2 +2 (IV) complexes were synthesized from 2-aminopyridine with 2,4 and 2,5 dihydroxy acetophenones. The Schiff bases and complexes were characterized by elemental analysis and spectral data. The Schiff bases as well as metal complexes of La (III), Sm (III), Th (IV) and UO2 +2 (IV) have been screened for their antimicrobial activity against Bacillus Subtilis, Escherichia coli, and Aspergillus flavis respectively. The comparision of antimicrobial activities of the ligands and metal complexes shows that the presence of metal causes more inhibition ie more activity.