The main objective of our research work is to prepare Ornidazole azo adduct with 5-ASA for colon targeting . We synthesized Ornidazole 5-ASA azo adduct and examine the effect of enzyme azo reductase on the release rate of ornidazole and 5-ASA in the gastrointestinal contents of rats. By using this approach two drugs can be targeted at the same time in the colon so as to treat the various disease of colon such as Crohn’s disease and ulcerative colitis. The azo adduct did not release drug in acidic environment of stomach, but when the azo adduct drugs will enter into colon the enzyme azo reductase break the azo bond and releases the dual drugs. By using this approach two drugs can be released at same time in colon .The azo adduct was evaluated for its color, solubility, Rf value, melting point and IR spectral analysis. It was further subjected for evaluating its colon targeting property by in-vitro method using rat fecal matter.