In the past two decades, a wide variety of bioactive peptides have been discovered.Many of the heterocyclic found to exhibit antifungal, antibacterial, cytotoxic, antineoplastic, insecticidal, anti-inflammatory, tyrosinase inhibitory and melanin production inhibitory activities.Imidazole has been drawn as promising structural units in the field of medicinal chemistry. Introduction of D-amino acids and N-methylation of amino acids like tyrosine, valine, alanine etc enhanced antimicrobial activity.The new phenyl alanine derivatives compounds are characterized by using IR, H1NMR and mass spectroscopy.