A novel series of 2-(5-substituted)-1,3,4-oxadiazole-2-yl)-H-imidazo[1,2,α]pyridine derivatives 5a-i and 6a-d are synthesized and characterized by IR, 1H NMR, 13C NMR and Mass spectral analysis. All the synthesized compounds were tested for there antibacterial and antifungal activity of which compound 5b, 5c, 5d, 5e, 5f, 5g, 5h, 5i, 6a, 6c and 6d exhibited good antimicrobial activity