In the present study, six substituted hydroxytriazenes were synthesized and confirmed by their elemental analysis. These synthesised hydroxytriazenes were screened for their antibacterial and antifungal activities. These compounds have shown very encouraging results showing inhibition zone from 15 mm to 45 mm against four bacterial and two fungal stains. Thus the study has brought about a novel application of this class of analytical reagents as an emerging class of bioactive chemicals