The synthesis and biological evaluation of some novel pyrazolo [3,4-d] pyrimidines was aimed at creating a new scaffold. Considering previous articles activity profile of pyrazolo [3,4-d] pyrimidines, seven new 4-(4-substituted benzoylamino) pyrazolo[3,4-d]pyrimidines have been synthesized by reacting different 4-substituted benzoylchlorides with 4-amino pyrazolo [3,4-d] pyrimidine in DMF solvent. All these compounds were characterized by physical and spectral data. These compounds were screened for anti-inflammatory activity by carrageenan induced edema model. Among the compounds tested for anti-inflammatory activity, Electron withdrawing groups such as chloro 6b and fluoro 6d substituted compounds showed appreciable results at 3mg/kg when compared with the standard drug indomethacin at10mg/kg respectively. Electron releasing groups such as methyl 6c and amino 6e substituted compounds showed inferior activity when compared to 6b and 6d derivatives.