A series of thiophene appended substituted pyrimidin-2-one analogues were synthesized by an accessible approach. The reaction of chalcones 1(a-e) and urea 2 in the presence potassium hydroxide in ethanol yielded 6-aryl-4- (thiophen-2-yl)-5,6-dihydropyrimidin-2(1H)-ones, 3(a-e) in good yields. The synthesized new compounds were characterized by 1H NMR, 13C NMR, Mass spectral studies and elemental analysis and were screened for their antifungal susceptibility against different fungi species.