A series of novel 1, 2, 4-oxadiazole derivatives were synthesized and evaluated for in- vitro antibacterial and antifungal activity. All the compounds tested against bacteria showed comparable or less antibacterial activities than the reference drug. Differences in their activity depend on the substitution of different groups. More specifically, best antibacterial activity among synthetic analogues was shown by compound 6 with MIC of 10 μg/ml and compound 14 with MIC of 50 μg/ml against Bacillus subtilis. All compounds tested showed significant antifungal activity against all the fungi, compared to the commercial fungicide Miconazole. Among them compounds 4 and 8 showed good antifungal activity against Fusarium solani with MIC of 10 and 50 μg/ml