Urtica dioica (UD) has been used as antihypertensive, antihyperlipidemic, anti antioxidant and anti diabetic herbal medicine. The purpose of this study was to study the effect of the diabetic rats of a swimming workout session along with the use of aqueous extract of Urtica dioica on histochemical properties of liver in rats.In this experimental study, 54 male Wistar rats injected with streptozotocin after induced diabetes as to injected intraperitoneal were randomly divided into 9 groups including healthy control , diabetes control, metformin at a dose of 100(gavage), diabetes and swimming, metformin and swimming, diabetes and UD aqueous extract at a dose of 1.25, diabetes and UD aqueous extract at a dose of 0.625(gavage), diabetes and UD extract dose of 1.25 and swimming, diabetic and UD extract at dose of 0.625(mg/kg/day) and swim. Swim training protocol was performed for 4 weeks, 5 days per week. Blood Aspartate aminotransferase (AST), alkaline phosphatase (ALP), Alanine amino transferase (ALT) were determined. ALP levels in diabetic groups and swim training, metformin and swim training, diabetes and UD extract at a dose of 0.625 and diabetes with UD extract at a dose of 1.25 and swim training with diabetic control group had a significant decrease(p-value of the respectively 0.02, 0.03, 0.01, 0.02). ALT, AST and metformin levels compared to diabetic control group were not significantly different. The present results suggest that swim training with UD extract on liver index was dose-dependent and also has synergistic effects on the liver factors in rats with hyperglycemia.