Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2016 Vol: 8 Issue: 6

Study the Antimicrobial Activity of Alcoholic Extract of Propolis and Balsam against a Imipenem-Resistant Pseudomonas Aeruginosa

Abstract

Propolis and balsam these two substances known to be curative. In ancient times they have been used for wound healing skin. In this study the antimicrobial activity of alcoholic extract of propolis and balsam against a imipenemresistant Pseudomonas aeruginosa have been examined 50 .Pseudomonas aeruginosa in a period of 4months from February to May 2014, were isolated. The strains from different clinical samples such as blood, urine, pus, etc., were isolated using microscopic and biochemical tests such as catalase, oxidase, the growth of the TSI, the response of the OF, grown on agar cetrimide ability to grow at 42degrees C were identified. The antibiotic susceptibility testing against 10different antibiotics using the disk diffusion method was used. The 50isolates of Pseudomonas aeruginosa resistance patterns of the isolates tested by disk diffusion antibiogram were as follows:Imipenem (30%), ampicillin (75%), tetracycline (70%), cephalothin (42.5%), cefotaxime (47.5%), amikacin (52.5%), nalidixic acid (45%), gentamicin (50%) ciprofloxacin (40%), cotrimoxazole (65%). Antimicrobial activity of the extracts was carried out by the agar dilution of the test minimum inhibitory concentration of propolis extracts against imipenemresistant Pseudomonas aerogenusa 125 to 500 micrograms per milliliter variable. The results of this study, the use of propolis and balsam in the treatment of skin infections and scars suggested course of these substances can be used in the prevention and treatment of skin diseases.