Sports injure can affect the training effect and performance of football players, therefore it is necessary to find out an effective treatment for it, the application of gene therapy on treating the sports injure of football players is studied in depth. First, the common sport injures of football player are summarized. Second, the reasons of football sports injure are analyzed. Thirdly, the basic idea of gene therapy is described. Then experimental analysis is carried out for 120 cases of football sports injure, and results show that the gene therapy can get better treating effect than traditional treatment.