There are various gall causing agencies in nature. Present study was thus undertaken on biochemical changes in leaf galls of Pongamia due to infection of Aceriapongamiae. The parameters assayed were total protein, total auxin content, free auxin content, IAA oxidase and invertase activity compared to normal tissues. Quantitative estimation of these metabolites and enzymes showed distinct variations in leaf gall at different ages with their normal counterparts.