Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2014 Vol: 6 Issue: 5

Study of separation and flocculation characteristics of a bioflocculant MBF B31


A bioflocculant named MBF B31 produced by strains B31 was investigated in this study. The bioflocculant MBF B31 was separated by organic solvent precipitation, the production yield of crude bioflocculant by cold ethanol precipitation was up to 0.7g/L, relatively high. Crude bioflocculant was purified using Sephacryl S-200 gel filtration chromatography, there was only a large single absorption peak at 216,280nm, the flocculating rate of the purified bioflocculant was 90.21% and increased very little compared with the crude bioflocculant. According to UV and IR spectra, colorimetric reaction, chemical composition of the bioflocculant was analyzed, the main ingredient of the bioflocculant was glycoprotein. Four factors influencing flocculating rate including bioflocculant dosage, pH, type and amount of metal ions were investigated, flocculation conditions were 10mg/L bioflocculant dosage, pH 8, CaCl2 as coagulant, 5mL CaCl2