Herbal medicinal products are essential in medical practice due to component composition that determines their maximum effect. Peculiarities of the determined substances action depends on the technology of plant extracts. The aim of our work was studyoflipophilicandhydrophilicfractionsofCO2calamusrhizomeextract. Obtained results indicate the presence of terpenoid, steroid nature substances, aromatic and oxygenated heteroaromatic compounds in a rhizomes Calamus CO2 extract.