Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2010 Vol: 2 Issue: 5

Studies on the production of protease from agro residues Convalia ensiformis beans by solid state fermentation (SSF) using Bacillus subtalis 2724NCIM

Abstract

The agro wastes are being dumped into the domestic environment causing unhygienic to the residents, and foul odor is becoming a common problem. Most of the agricultural wastes are contributing solids wastes, which are becoming hazardous to the public. By utilize the agro wastes for the bioconversion process we can reduce the environmental pollution problems. Convalia ensiformis beans have low nutritional quality and high content of crude fibre in the seed coat. Hence these beans are being neglected for consumption. In the present study an attempt was been made to utilize these beans for the production of protease enzyme by solid state fermentation (SSF) using bacillus subtilis2724NCIM.The study indicated, these beans were more suitable for the production of protease, which showed an activity of 680μg/ml.Various parameters like optimum pH, temperature, substrate concentration, effect of carbon and nitrogen source were studied. The study indicated the best utilization of agro wastes for the production of protease enzyme which has wide applications in the field of food and pharmaceutical industries. These bioconversion studies of agro waste contributed the management of environmental safety and health by effective utilization of agro residues.