To purify the platycodins by AB-8 macroporous resin, the adsorption isotherms and adsorption kinetics were studied. The effect of loading sample process on breakthrough curves of platycodins in AB-8 resin fixed-bed was also investigated. The result show that the concentration of loading sample was 2.0 mg/mL, the elution velocity of flow was 2.0 mL/min and the concentration of eluant was 80% ethanol. The product purity was 92.13% with a recovery rate of 78.41% on this condition. The research results provided a suitable method for puritying platycodins.