Objective: The research has a threefold objective: Synthesizing and characterizing AgNPs, investigating their impact on blood contaminated by mercury toxin, and examining AgNPs binding with bio-coated red blood cells.

Methods: This study explores the green synthesis of Silver Nanoparticles (AgNPs) using neem and Aloe vera leaves, characterized through UV-vis spectrophotometry and scanning electron microscopy.

Results: AgNPs couldn't prevent mercury-induced hemolysis, they effectively bound to RBCs in blood groups B+ and O+, with partial hemolysis in A+. These findings suggest potential biomedical applications.

Conclusion: This research contributes to the broader fields of nanotechnology, biotechnology, and environmental science, providing valuable insights into the potential of green-synthesized AgNPs and addressing the urgent need for innovative solutions to mercury contamination and its impact on human health and the environment.