In the present study of ethanolic extract of leaf of Thespesia populnea Linn. (malvaceae) was screened for anti-inflammatory activity an carrageenan induced paw oedema rats. The effect was assessed by difference in paw cedema volume, before and after the low and high dose administration of the extract in rats. Ethanolic extract of Thespesia populnea leaf (100mg/kg body weight) were administered orally. The acute toxicity studies of oral doses of ethanolic extract of Thespesia populnea leaf in rats revealed that it has a high safety profile, as the extract (100mg/kg body weight) was well tolerated by the animals. The results of the study suggest that the extract possesses anti-inflammatory activity.