In the few decades, there has been exponentially growth in the field of herbal remedies. Newer guidelines for standardization, manufacture, quality control and scientifically rigorous research will be necessary for traditional treatments. This traditional knowledge can serve as the powerful search engine that will greatly facilitate drug discovery. Standardization of herbal formulations is essential in order to assess the quality, purity, safety and efficacy of drugs based on the amounts of their active principles. The aim of the present work is to standardize Gokshuradi churna. The churna makes this traditional drug more stable for long term storage and hence, easier to prepare. The “Gokshuradi churna” an Ayurvedic polyherbal formulation used in the treatment of is a polyherbal Ayurvedic medicine used as a diuretic and cardiac tonic. One marketed and one in- house formulations were used for the study. All the formulations were standardized on the basis of organoleptic characters, physical characteristics and physico-chemical properties. The set parameters were found to be sufficient to evaluate the churna and can be used as reference standards for the quality control/quality assurance purposes. The analysis and quality control of herbal medicines are moving towards an integrative and Comprehensive direction, in order to better address the inherent holistic nature of herbal medicines. It was observed that all ingredients of commercial samples matched exactly with that of authentic standards after performing the standardization as per WHO guideline.